SUBSTANTIATION OF THE PLACE OF ALTERNATIVE BIOLOGICAL METHODS IN HYGIENIC RESEARCH
Introduction. The article presents an analysis of the most common methods of biotesting, as well as the search for promising directions for the development of rapid diagnostics. The advantages of alternative biological methods for assessing chemical pollutants in environmental objects to study their effect on living organisms are considered. Material and methods. The paper compares data on the toxicity of various anti-ice materials, ash and slag pharmaceutical preparations and pyrolysis mixture of filtration combustion in experiments using animals and hydrobionts. Ecotoxicological studies were carried out using daphnia, infusorium, luminous bacteria, as well as the culture of mammalian cells. Toxicological - in acute, subacute experiments, including the study of specific immunological indicators, allergenic and skin-irritating effects. Results. For the toxicants studied, inactive concentrations and dilutions are determined for biological models, hazard classes are established. The results of toxicological experiments are presented. Discussion. Attempts to improve the methods of biotesting are carried out in the direction of the creating a battery of bio-tests, shortening the terms of testing and instrumentalizing studies. The analysis of works on various areas of biological research allows us talking about the need to use alternative methods in the preliminary stages of obtaining results. This may be applicable to the establishment of MPC of chemicals in the soil. For each of the indices of harmfulness can be used as a preliminary biotest: the survival of daphnia - for the water-migration index, the presence of Azotobacter - for general health, etc. Conclusion. The selectivity of the responses to toxicants makes it possible to identify target organs in warm-blooded animals, and also to predict the impact on environmental objects through hydrobiont models.
About the authorsDonerian Larisa G.
Viana, L.F., Súarez, Y.R., Cardoso C.A.L. et al. Arch Environ Contam Toxicol. 2018; 75: 476. https://doi.org/10.1007/s00244-018-0551-9.
Zaritskaya E.V., Polozova E.V., SHilov V.V., Bogacheva А.S. Present alternative study methods used in product safety assessment. Ehkologiya cheloveka; 2017; 3: 21-25 (in Russian).
Lazarev N.V. Levina EH.N., ed. Harmful substances in industry. A Handbook for Chemists, Engineers and Physicians; 1976; 1; Organic matters: 592 (in Russian).
Pozharov А.V., Rakhmanin YU.А., SHelemotov S.А., Mikhajlova R.I. Applied aspects of instrumental biotesting of water. Gigiena i Sanitaria. 1994; 8: 18-21 (in Russian).
Krasovskij G.N., Rakhmanin YU.А., Egorova N.А.: Extrapolation of toxicological data from animals to humans: Moscow: Meditsina Publishers, 2009. 208 p. (in Russian).
Krasovskij G.N., Egorova N.А. Disadvantages of biotesting in the hygienic assessment of wastewater. Gigiena i Sanitaria. 2005; 3: 10-13 (in Russian).
Bal’buhov K.S., Kapranov S.V. Environmental and hygienic assessment of the quality of industrial wastewater and open reservoirs in an industrial city using the biotesting method; Water and water treatment technology. Scientific and Technical Fields. 2012; 8 (2): 30-39 (in Russian).
Matorin D.N., Bratkovskaya L.B., Yakovleva O.V., Venediktov P.S. Biotesting of water toxicity according to the ratio of microalgae consumption by daphnia detected with chlorophyll fluorescence. Moscow University Biological Sciences Bulletin. 2009; 64 (3): 115-120. DOI: 10.3103/S0096392509030067.
Mushunina A.S., Azarova S.V., Yazikov E.G., Parygina I.A. Biotesting of modeled drilling mud as an indicator of environmental risk; IOP Conf. Series: Earth and Environmental Science. 2016; 43. DOI: 10.1088/1755-1315/43/1/012047.
Terekhova V.А. Soil biotesting: approaches and problems. Pochvovedenie. 2011; 2: 190-198 (in Russian).
Mayachkina N.V., Chugunova M.V. Peculiarities of soil biotests to evaluate soil ecotoxicity. Vestnik Nizhegorodskogo universiteta im. N.I. Lobachevskogo. 2009; 1: 84-93 (in Russian).
Rusakov N.V., Mukhambetova L.KH., Kryatov I.А., Koganova Z.I., Fadeeva I.I., CHudakova S.B. and al. Assessment of the degree of danger of chemicals polluting the soil when exposed to the body of experimental animals. Gigiena i Sanitaria. 2007; 2: 68-69 (in Russian).
Evseeva I.S. Ecological and hygienic assessment of the thermal technology for the destruction of medicinal products subject to disposal; Dis…. kand. med. nauk. M. 2006: 204 (in Russian).
CHudakova S.B. Toxicological and hygienic evaluation of the degree of danger of ice-reagents; Dis…. kand. med. nauk. M. 2006: 191. (in Russian).
Olkova A.S. Bioassay in research and environmental practices of Russia. Uspekhi sovremennoj biologii. 2014; 134 (6): 614-622. (in Russian).
Evsyunina E.V., Taran D.O., Stom D.I., Saksonov M.N., Balayan A.E., Kirillova M.A., Esimbekova E.N., Kratasyuk V.A. Comparative assessment of toxic effects of surfactants using biotesting methods. Inland Water Biology. 2016; 9 (2): 196 - 199.
YAkovishina T.F. Ecotoxicological assessment of urban soils using biotesting methods; Universum: Chemistry and Biology: electronic science. 2015. №8 (16). URL: http://7universum.com/ru/nature/archive/item/2491 (appeal date: 13.11.2018) (in Russian).
Jonczyk E., Gilron G. (2005) Acute and Chronic Toxicity Testing with Daphnia Sp. In: Blaise C., Férard JF. (eds) Small-scale Freshwater Toxicity Investigations. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3120-3_11.
Аrystarkhova Е. Express-assessment of potential hazard of water by method of biotesting using Daphnia magna S. Bulletin of Agrarian Science; 2017; (2): 50-54.
Rykunova M.D., Nelyubova V.V., Karnaukhova M.D. Ecotoxicological studies of bioactive components; Innovations in construction - 2017; Publishing house: FGBOU VO “Bryansk State Engineering and Technology University” (Bryansk). Materials of the International Scientific and Practical Conference Innovations in Construction; 2017; Bryansk, November 20-22, 2017: 121-126 (in Russian).
Krasovskij G.N., Egorova N.А. Methodological errors in the use of biotesting in hygiene studies. Gigiena i Sanitaria. 2000; (4): 63-66 (in Russian).
Dolgov V.А. Methodical aspects and practical application of the accelerated biological assessment of fodder, livestock products and other objects of veterinary-sanitary and ecological control. Аvtoref. Dis. …d-ra nauk. M. 1992: 41 (in Russian).
Dolgov V.А., Lavina S.А., Nikitchenko D.V. Comparative evaluation and relationship of toxicity parameters of different substances to Tetrahymena pyriformis and white rats. Vestnik rossijskogo universiteta druzhby narodov. Series: agronomy and livestock; 2014; 2: 58-65. (in Russian).
Dolgov V.A., Lavina S.A., Arno T.S., Semyonova E.U., Ostrovskaya A.V. Bioassay of quality and safety of feed, food and the environmental objects. Rossijskij zhurnal. Problemy veterinarnoj sanitarii, gigieny i ehkologii. 2015; 2 (14): 20-27 (in Russian).
Dolgov V.A., Lavina S.A. Methodological aspects of veterinary-sanitary expertise of food raw materials and food products. Rossijskij zhurnal. Problemy veterinarnoj sanitarii, gigieny i ehkologii. 2016; 3(19): 11-19 (in Russian).
Strizhak T.А., Sidashova S.А., Palaguta А.V. The method express research on toxicological profile of feed base of pig farms. Nauchno-tekhnicheskij byulleten’ instituta zhivotnovodstva nacional’noj akademii agrarnyh nauk Ukrainy; 2016; 116: 151-160 (in Ukrainian).
Baumann, J., Sakka, Y., Bertrand C. et al. Environ Sci Pollut Res. 2014; 21: 2201. https://doi.org/10.1007/s11356-013-2094-y.
Ignace D.D., Dodson S.I., Kashian D.R. Identification of the critical timing of sex determination in Daphnia magna (Crustacea, Branchiopoda) for use in toxicological studies. Hydrobiologia. 2011; 688 (1): 117-123.
Robinson S.E., Capper N.A., Klaine S.J. The effects of continuous and pulsed exposures of suspended clay on the survival, growth, and reproduction of Daphnia magna. Environmental Toxicology. 2010; 29 (1).
Zakharov I.S., Aleshin I.V. Methods and Means for Microscale Biotesting Toxicity of Aquatic Environments. Fundamental and applied hydrophysics. 2015; 8 (2): 75-95 (in Russian).
- Refbacks are not listed
Контент доступен под лицензией Creative Commons Attribution 3.0 License.