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THE ASSESSMENT OF THE ANTIOXIDANT STATUS OF THE ORGANISM OF RATS UNDER THE CHRONIC INTAKE OF SURFACE-ACTIVE SUBSTANCES BASED ON THE RAPE OIL


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Abstract

Introduction. The study of the character of reactions of lipid peroxidation, as well as the state of the antioxidant system regulating their course under the conditions of the action of chemical products on the body, is an important stage of investigations. There is not enough data on the development of oxidative stress in cells exposed to pesticides, and this concerns, first of all, studies of the activity of antioxidant enzymes. The aim of the study is to execute the experimental assessment of the influence of a surface active substances (SAS) on the basis of the rape oil on antioxidant status of white male rats during its multiple peroral intakes for 6 months in doses of 0.5, 5.0 and 50.0 mg/kg body weight (on a.s.). Material and methods. Studies on the determination of enzymatic indices of the body's antioxidant defense system (superoxide dismutase, glutathione peroxidase and glutathione reductase) were performed on a ChemWell biochemical analyzer (Awareness Technology, USA) with the use of diagnostic reagent kits by Randox Laboratories Ltd (England). The catalase activity was determined by the colorimetric method. Results. Under the prolonged action of surfactants on warm-blooded organisms, changes in the activity of antioxidant enzymes were established to occur in doses of 5,0 and 50,0 mg/kg b.m. Discussion. The test substance used in a dose of 0.5 mg/kg bm does not have an inhibitory effect on the antioxidant status of the organism. The use of a surfactant based on rapeseed oil at doses of 5.0 and 50.0 mg/kg mt has a negative effect on the antioxidant status of an organism of male rats. Conclusion. Surfactants based on rapeseed oil in doses of 5.0 and 500 mg/kg b.m. have a negative effect on the antioxidant status of an organism of male rats, which is probably due to the high content of erucic acid and glucosinolates in rapeseed oil.


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