COMPARATIVE ASSESSMENT OF THE SANITARY AND EPIDEMIC IMPORTANCE OF СOLIFORM INDICATORS OF THE DRINKING WATER QUALITY
Introduction. The criteria used for the sanitary-bacteriological assessment of the quality of tap water should ensure its epidemic safety, which is important when choosing priority indicator microorganisms, quantitative determination of which will allow characterizing the risk of intestinal infections associated with water use conditions with sufficient reliability. Material and methods. Analyzed water studies for 10 years for the presence of sanitary-indicative bacteria in the water of surface water bodies in accordance with the guidelines MUK 4.2.1884. To isolate and quantify Salmonella, there were used the conventional magnesium medium and the developed nutrient medium, ready for use. The obtained data were processed using the statistical software package Statistic for Windows. Results. It has been established that E.coli can be considered as an additional indicator in order to assess recent fecal contamination. The absence of E.coli in drinking water does not guarantee the absence of other members of the family Enterobacteriaceae. Pathogenic and potentially pathogenic bacteria exhibited the greatest viability. E. coli turned out to be less stable, i.e. microorganisms, on an indication of which the system of sanitary and epidemic safety of the water use was built. In the experiments, lactose-positive coliform bacteria were established to be less resistant to the action of disinfecting agents than salmonella and potentially pathogenic bacteria, which should not be inherent to the indicator microorganism. Conclusion. Research results indicate that the absence of normalized lactose-positive fecal indicators fails to guarantee the absence of infectious agents since lactose-negative enterobacteria are unaccounted for - pathogenic and potentially pathogenic species causing intestinal infections. Therefore, in the sanitary-bacteriological control of tap water, it is more expedient to focus on the indicator glucose coliform bacteria (GKB).
About the authorsRakhmanin Yu.A.
Zhuravlev Piotr V.
SanPiN 188.8.131.529-96. “Drinking water. Hygienic requirements to water quality of centralized drinking water supply systems. Quality control”. M., 1996.
SanPiN 2.1.4 1074-01 Drinking water. Hygienic requirements to water quality of centralized drinking water supply systems. Quality control”. M., 2001.
GOST 2874-73 “Drinking water.” M., 1973. 16 p.
GOST 18963-73 “Drinking water. Methods of the sanitary and bacteriological analysis”. M., 1986. 13 p.
Aleshnya V. V., Zhuravlyov P.V., Golovina S.V., etc. Value of indicator microorganisms at an assessment of microbic risk in emergence of epidemic danger at drinking water use. Gigiena i sanitariya.2008 (2): 23-6.
Artemova T.Z., Turchinsky I.N., Chernavskaya N. V. To a question of reliability of a laktozny sign at quality control of drinking water. Tezisy dokladov Vsesojuznoj konferencii po sanitarnoj mikrobiologii. M., 1978: 180-1.
Zhuravlev P.V, Aleshia V.V., Golovina S.V., etc. Monitoring of bacterial pollution of reservoirs of the Rostov region. Gigiena i sanitariya. 2010 (5): 33-5.
Dechesne M., Soyeux E. Assessment of source water pathogen contamination. Journal of Water and Health.2007; 5 (1): 39-50.
State report “On the state sanitary and epidemiological welfare of the population in the Russian Federation (2000 - 2010).
Zhuravlev P.V, Aleshia V.V., Panasovets O.P., etc. Value glyukozopolozhitelnykh koliformnykh of bacteria and potentially pathogenic bacteria as indicators of epidemic safety of tap water. Gigiena i sanitariya. 2013 (1): 56-8.
Nedachin A.E., Artemova T.Z. “Actual problems of preventive medicine, quality management of habitat and health of the population. Materials of scientific and practical conference”. Cherepovets. 2004: 63-72.
Nedachin A.E., Artemova T.Z., Dmitriyeva R. A., etc. Problems of epidemic safety of drinking water use of the population of Russia. Gigiena i sanitarija. 2005 (6): 14-8.
Nedachin A.E., Artemova T.Z., Talaeva Y.G, Ivanova L.V., Bogatyreva I.A., Butorina N.N., Zagainovа A.V. Improvement of normative and methodical base of bacteriological drinking water quality monitoring. Gigiena i sanitariya. 2007 (5): 36-9.
Nedachin A.E., Artemova T.Z., Talayeva Yu.G., etc. Comparative value of indicator bacteria in an assessment of potential danger of developing of intestinal infections at drinking water use. Results and prospects of scientific researches on an environmental problem of the person and hygiene of environment. M., 2005: 48-63.
Barrell R. A., Hunter, P.R., Nichols G. Microbiological standards for water and their relationship to health risk. Commun. Dis. Public Health. 2000; 3 (1): 8-13.
Council Directive of the European Union 98/83/EC of 3.11.98 quality of water intended for human consumption. M. 1999.
Gray N.F. Drinking water Quality. Problems and solutions’. 1994.
Craun U.F., Nwachuku N., Calderon R.L., Craun M.F. Outbreaks in drinkingwater systems, 1991-1998. J. Environ. Health. 2002; 65 (1): 16-23.
Methodical Instructions.184.108.40.2068-01 “The sanitary and microbiological analysis of drinking water”. M., 2001. 42 p.
GOST 31955-2012 (ISO 9308-1:2000) “Drinking water. Detection both quantitative accounting of Escherichia coli and coliform of bacteria”. Part 1. “Method of a membrane filtration”.
Methodical Instructions.4.2.1884-04 “Sanitary and microbiological and sanitary and parasitological water analysis of superficial water objects”. M., 2004. 75 p.
Nutrient medium for the accumulation of Salmonella, ready-to-use (TU 9385-001-01898776-2008, PN No. SDF 2009/05759 from 29.09.2009). Rostov-on-Don. 2012.
Methodical Recommendation 01-19/98-17 “An advanced method of detection of Enterobacteria and the Microorganism which aren’t fermenting the Gramm negative in objects of the water environment”. Rostov-on-Don, 1996.
Methodical Recommendation 4116-86 “Methodical recommendations about a hygienic regulation of microbes pollution”. M., 1986. 13 p.
GOST 31942-2012 (ISO 19458:2006) “Drinking water. Sampling for the microbiological analysis”.
Maleta Yu.S., Tarasov V. V. Nonparametric methods of the statistical analysis in biology and medicine. Publishing in Moscow University. M., 1982. 178 p.
The determinant Bergey. Ed. by Acad. RAS G.A. Zavarzin. M., 1997; 1: 430.
- Refbacks are not listed
Контент доступен под лицензией Creative Commons Attribution 3.0 License.