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SURVIVAL OF BACTERIA IN A SIMULATION OF SURROUNDING SURFACES OF CONSTRUCTIONS FOR SALT THERAPY


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Abstract

Introduction. Ground constructions for salt therapy, which differ in the composition of minerals and in the modification of therapeutic surfaces, are actively used in Russia and abroad. The abiotic surfaces of these devices are susceptible to microbial contamination, the sources of which are the upper respiratory tract, the skin of patients and medical staff. The aim of the work is to assess the viability of microorganisms on abiotic surfaces identical to the material for the manufacture of salt physiotherapy constructions. Material and methods. 6 and 24 hours after application to abiotic surfaces with different relief there was evaluated the survival rate of reference gram-positive and gram-negative cultures of microorganisms and isolated ones from salt rooms. Viable cells were determined by the method of decimal dilutions in terms of the number of colonies-forming units (CFU). Results. It was found that bacterial cells can preserve at the halite and various surfaces of sylvinite for at least 24 hours. The viability of microorganisms was independent of the ratio of minerals (halite/sylvin) in the samples. The structure of the surface of sylvinite: the largest number of microorganisms was retained on crushed sylvinite (7.98E+02 ± 1.62E+03 CFU/ml was a significant factor for the survival of bacteria. Despite a great survival of staphylococci in comparison with gram-negative opportunistic pathogenic bacteria on all the surfaces studied, no significant differences between the groups were detected. In this case, bacteria isolated from salt structures were more resistant to salt load due to adaptive modification of microorganisms, including increasing the hydrophobicity of the cell wall, increasing their ability to survive. Bacteria grown on a solid agar medium proved to be more tolerant of the conditions of osmotic stress. Conclusion. The obtained data confirm the dependence of the adaptive mechanisms on the environmental conditions and the initial physiological state of cells. The results of studies on the survival of bacteria on salt surfaces of various types indicate their resistance to high concentrations of salts, which raises the question of special methods for treating fences of salt therapy structures.


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