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ANALYSIS OF CHANGES IN CASP7 AND CHEK1 GENES EXPRESSION IN LIVER TOXIC DAMAGE INDUCED BY TETRACHLORMETHANE AGAINST THE BACKGROUND OF HEPATOPROTECTORS


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Abstract

Introduction. The effect of hepatoprotectors on the expression of genes involved in the regulation of apoptosis and the cell cycle (Casp7 and Chek1) under the toxic effects of carbon tetrachloride (carbon tetrachloride, CCl4) was studied. Material and methods. Studies were performed in male albino mongrel rats (n=70), assigned to the control group, modulated with CC14-induced toxic hepatitis and three experimental ones in which the administration of drugs (Heptor, Mexidol and Methyluracil) was carried out 1 hour before CCl4 administration. After 24 and 72 h of CCl4 administration animals were decapitated and the liver was removed. The expression of Casp7 and Chek1 genes in rat liver was studied by real-time using polymerase chain reaction with reverse transcription. Results. Toxic damage to the liver of rats receiving CCl4 after 24 h resulted an increase in the expression of the Chek1 gene. The use of hepatoprotective drugs practically did not alter the expression of the Casp7 and Chek1 genes relative to the group that received only CCl4. At the same time, when comparing the effect of Mexidol, there were significant differences in the expression of the genes under study between two time points of 24 and 72 h. The effect of Heptor revealed changes in the expression level of the Casp7 gene when comparing the results 24 and 72 h after administration. Conclusion. The administration of hepatoprotectors to animals receiving CCl4 did not significantly affect the transcriptional activity of the Casp7 and Chek1 genes.


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