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Isolation of highly purified plasma factor IX


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Abstract

The new laboratory method for isolation of highly purified concentrated factor IX (FIX) includes a series of stages - ion exchange, affinity, and immobilized metal ion affinity chromatographies. Optimal conditions for affinity and immobilized metal ion affinity chromatography have resulted in FIX output of 83±10 and 82±7%, respectively. The resultant concentrate with FIX specific activity of 19.6±5.5 U/ml contains no more than 0.0001, 0, and 0.0004 U FII, FVII, and FX per unit of FIX. The results can be used for developing a technology for obtaining highly purified concentrated FIX from human plasma.


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