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CHANGES OF PLATELET ACTIVATION MARKERS IN DONOR PLATELETS CONCENTRATES DURING THE STORAGE AFTER THE INACTIVATION OF PATHOGENS BY AMOTOSALEN AND ULTRAVIOLET A RADIATION TECHNOLOGY


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Abstract

Pathogen inactivation treatment of platelet concentrates improves the transfusion safety. The influence of the pathogen inactivation on the platelet activation remained unclear. The aim of the study was to evaluate the changes of the platelet activation markers in donor platelets concentrates during the storage after the inactivation of pathogens by amotosalen and ultraviolet A. By flow cytometry CD62P (P-Selectin) and activated GPIIb/IIIa surface expression were estimated with a mouse-anti-human IgG1 CD62-PE antibodies and mouse-anti-human GPIIb/IIIa-FITC (PAC-1) antibodies. In platelet concentrates during the storage the fraction of the spontaneously activated platelets increased and fraction of the platelets capableof specific activation under the action of the ADP agonist decreased. The pathogen reduction did not affect the ability of platelets to specific activity, but led to an increase in the fraction of the spontaneously activated platelets. Replacement of plasma with a solution SSP+ led to the increase in the proportion of spontaneously activated platelets, but during prolonged storage has contributed to the preservation of the fraction of functionally active platelets.


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