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HIGH-THROUGHPUT IMMUNOGLOBULIN SEQUENCING DATA ANALYSIS WITH THE USE OF UNIQUE MOLECULAR IDENTIFIERS


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Abstract

High-throughput sequencing made deep analysis of immune receptors repertoires possible. However, B cells’ ability to accumulate hypermutations indistinguishable from PCR errors along whole variable domain and low quality of extended sequencing still impose a serious obstacle to analysis of full-length variable immunoglobulin sequence repertoires. In present review we demonstrate how molecular barcoding technique (unique molecular identifiers, UMI) and bioinformatics analysis provide nearly error-free immunoglobulin repertoires profiling of complex B-cell populations comprising minor hypermutated subvariants.


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