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PHENOTYPIC CHARACTERISTICS OF BLOODBORNE CLASSICAL DENDRITIC CELLS AND THEIR SUBPOPULATIONS IN NORM AND IN OSTEOMYELITIS


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Abstract

The degree of maturity and the subpopulation composition of human bloodborne classical dendritic cells (DCs) were studied in norm and in chronic osteomyelitis. For this purpose, enriched samples of classical DCs were obtained from venous blood by magnetic separation and expression of markers was analyzed by laser flow cytometry. It is shown, that classical DCs from blood of adult healthy donors have a phenotype of immature DCs with extremely low level of CD83 expression and absence of CCR7. About half of blood DCs expresses CD86. The subpopulation of CD141+ DCs from healthy donors exhibits a less mature phenotype than CD1c+ subpopulation. CD141+ subpopulation contains significantly smaller amount of CD86+ cells, and the cells of this subpopulation have a lower level of HLA-DR expression (lower fluorescence intensity). The proportions of CD1c+ DCs and CD141+ DCs in patients with osteomyelitis and healthy donors do not differ, but the degree of maturity of DCs decreases in osteomyelitis, which manifests itself in a significant decrease in the number of CD83+ and CD86+ cells. Also, the degree of cell maturity is reliably reduced in CD1c+ subpopulation. CD83+ cells practically disappear in both DC subpopulations in osteomyelitis. Thus, the inflammatory process causes enrichment of the DC blood pool by the less mature cells, but does not lead to the appearance of mature DC in osteomyelitis.


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