MORPHOLOGICAL CHARACTERISTICS AND THE COMPOSITION OF TYMUS CELLS IN EXPERIMENTAL ACUTE ULCERATIVE COLITIS OF DIFFERENT SEVERITIES
The morphological changes of thymus and its cell composition were studied in experimental acute colitis of variable severity - catarrhal-ulcerative and fibrinose-ulcerative, induced with 1% and 5% water solution of sodium dextran sulfate salt. Annexin V-positive cells, which die by apoptosis, were identified in the thymus using flow cytofluorimetry. The chromogenic LAL-test was used to evaluate the endotoxin level in the blood serum. Compared to the control group the serum endotoxin level was 3.9 time higher in the mice with catarrhal-ulcerative colitis, and 9.6 times higher in the mice with severe fibrinose-ulcerative colitis. During acute catarrhal-ulcerative colitis in the adult male C57Bl/6 the accidental thymic involution is moderate, while in the mice with fibrinose-ulcerative colitis it is pronounced. During the moderate and severe accidental thymic involution the number of dying cells in the thymus increases 1.2 and 1.8 fold respectively, there is a decrease in the lymphocyte total number, CD3-CD19+ В-, CD3+CD8+ cytotoxic, Т-, CD4+CD8+ double positive lymphocytes, CD3+CD4+ Т helpers and CD4+CD25+Foxp3+ regulatory T lymphocytes, F4/80+ macrophages. During the moderate accidental involution the content of CD4-CD8-CD45+CD11c+dendritic cells decreases in the thymus and in the severe accidental involution there is an increase of the content of CD4-CD8-CD45+CD11c+dendritic and CD326+UEA+CD205-epithelial cells in the thymic medulla.
About the authorsPostovalova Ekaterina Andreevna
Tkachev A.V., Mkrtchyan L.S., Nikitina K.E., Volynskaya E.I. Inflammatory bowel disease: crossing of the problems. Prakticheskaya meditsina. 2012; 3(58): 17-22. (in Russian)
Powrie F. Immune regulation in the intestine: a balancing act between effector and regulatory T cell responses. Ann N. Y. Acad. Sci. 2004; 1029: 132-141.
Watanabe N., Wang Y., Lee H.K., Ito T., Wang Y., Cao W., Liu Y. Hassall’s corpuscles instruct dendritic cells to induce CD41CD251 regulatory T cells in human thymus. Nature. 2005; 436: 1181-5.
Garib F.Yu, Rizopulu A.P. Strategy of immune evasion of pathogens: the suppression of immune response by host’s T-regulatory cells activation. Immunologiya. 2016; 37 (1): 35-45. (in Russian)
Diatroptov M.E., Makarova O.V., Serebryakov S.N., Trunova G.V., Malaitsev V.V. Morphofunctional changes in the immune system of Balb/c and C57BL6 mice under the influence of a low dose of lipopolysaccharide. Immunologiya. 2011; 32(3): 135-8. (in Russian)
Sasaki S., Ishida Y., Nishio N., Ito S., Isobe K. Thymic involution correlates with severe ulcerative colitis induced by oral administration of dextran sulphate sodium in C57BL/6 mice but not in BALB/c Mice. Inflammation. 2008; 31: 5.
Ziegler S.F., Roan F., Bell D.B., Stoklasek T.A., Kitajima M., Han H. The Biology of Thymic Stromal Lymphopoietin (TSLP). Advanc. Pharmacol. 2013; 66.
Noble C.L., Abbas A.R., Cornelius J., Lees C.W., Ho G.T., Toy K. et al. Regional variation in gene expression in the healthy colon is dysregulated in ulcerative colitis. Gut. 2008; 57(10): 1398-405.
Charbonnier L.M., Wang S., Georgiev P., Sefik E., Chatila1 T.A. Control of peripheral tolerance by regulatory T cell-intrinsic Notch signaling. Nat. Immunol. 2015; 16(11): 1162-1173.
- Refbacks are not listed
Контент доступен под лицензией Creative Commons Attribution 3.0 License.