РАЗРАБОТКА И ВАЛИДАЦИЯ МЕТОДИКИ КОЛИЧЕСТВЕННОГО ОПРЕДЕЛЕНИЯ МАЛОНОВОГО ДИАЛЬДЕГИДА МЕТОДОМ ВЭЖХ-МС/МС

Разработана и валидирована биоаналитическая методика количественного определения малонового диальдегида (МДА) методом ВЭЖХ-МС/МС. Предложенный метод определения МДА включает стадию высвобождения связанного МДА без реакции дериватизации. Нижний предел количественного обнаружения составил 600 нмоль/л, объем необходимой пробы 10 мкл, время анализа 7 мин. Полученный в ходе исследования диапазон концентраций дает возможность использовать данную биоаналитическую методику для определения концентрации МДА в биологическом материале при оценке физиологических и патологических состояний.

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