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A COMPARATIVE STUDY OF EXPERIMENTAL AND COMMERCIAL SEROLOGICAL TESTS FOR DETECTION OF ANTIBODIES IN HUMANS WITH TULAREMIA


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Abstract

Experimental serological tests were developed to determine anti-tularensis antibodies in humans in immunochromatography formats (LF-test LPS Ft15) and enzyme immunoassay (ELISA LPS Ft15) using as an antigen highly purified LPS F. tularensis 15 NIIEG. Analysis was conducted of the sensitivity and specificity of the developed tests and commercial tularemia antigen «RNGA-Tul-AG» (production Stavropol research anti-plague Institute) in comparison with the commercial reference antigen, registered in the Russian Federation for the quantitative determination of human IgG tularemia - «ELISA classic Francisella tularensis IgG» SERION, Germany (IgG SERION ELISA). A study of human blood serum vaccinated against tularemia showed that the sensitivity and specificity of detection of anti-tularemia antibodies by «ELISA LPS Ft15» were 97.7 and 100%, compared with «ELISA IgG series». When determining anti-tularemia antibodies with the diagnosis «LF-test LPS Ft15», these parameters were compared to «ELISA IgG series» 94.3 and 100%. The sensitivity and specificity of «RNGA-Tul-AG» made compared to the «IgG ELISA, SERION» of 59.1% and 80%.


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