ALTERNATIVE AND PROMISING TARGETS OF BIOCHEMICAL ANALYSIS IN SPORT (REVIEW OF LITERATURE)
Current literature review provides an evaluation of advantages and limitations of biochemical control objects representing functional state of athletes as well as the outlook for using alternative targets regarding sports medicine. Traditionally, invasive procedures (venous blood collection, muscle biopsy) have been known as the gold standard for analyzing a wide range of biomarkers which could be employed as effective diagnostic tools to control the course of adaptation processes, monitor performance, overtraining and physical well-being of athletes, but these techniques are painful, time-consuming and place demands on storage and shipment. In this behalf finding an alternative objects for biochemical research that does not have disadvantages given above is the question of present interest. Saliva and dry blood spots (DBS) could serve as equally informative and promising targets for monitoring athletes’ condition. The non-invasive nature of saliva collection allows to shorten sample collection time, reduce stress hormones levels and possible infection contamination. Moreover, collecting saliva process does not require special equipment and trained medical staff which is particularly important when athletes are at training camps. The DBS method has successfully proven itself with regard to neonatal screening and pharmacokinetics studies. Its key benefits are simplicity, small volume of bioliquid, enhanced stability of adsorbed biomarkers on the card surface, lack of special storage and transportation requirements and low costs for samples shipment to the laboratory. Taken together outlined advantages will provide the opportunity to increase the frequency of biomaterial collection to perform selective observation of training loads effects on various systems of athletes’ body. The combination of DBS with immunochemical and mass-spectrometric approaches could serve as an efficient instrument to investigate the role of various biomarkers in monitoring the functional state of athletes. We searched for articles in MedLine database with the key words «dry blood spots», «saliva», «sports medicine», «sample collection», «sports biochemistry».
About the authorsDudko Grigoriy Alekseevich
Mosby. Mosby’s medical dictionary, 8th ed. St. Louis, Mo.: Elsevier; 2009.
Eastell R., Brandi M.L., Costa A.G., D’Amour P., Shoback D.M., Thakker R.V. Diagnosis of asymptomatic primary hyperparathyroidism: proceedings of the Fourth International Workshop. J. Clin. Endocrinol. Metab. 2014;99(10): 3570-9.
Weckesser L.J., Plessow F., Pilhatsch M., Muehlhann M., Kirschbaum C., Miller R. Do venepuncture procedures induce cortisol responses? A review, study, and synthesis for stress research. Psychoneuroendocrinology. 2014;46: 88-99.
Lindsay A., Lewis J.G., Scarrot C., Gill N., Gieseg S.P., Draper N. Assessing the effectiveness of selected biomarkers in the acute and cumulative physiological stress response in professional rugby union through non-invasive assessment. Int. J. Sports Med. 2015;36(6): 446-54.
Schneyer L.H., Young J.A., Schneyer C.A. Salivary secretion of electrolytes. Physiol. Rev. 1972;52(3):720-7.
Kreusser W., Heidland A., Hennemann H., Wigand M.E., Knauf H. Mono- and divalent electrolyte patterns, pCO2 and pH in relation to flow rate in normal human parotid saliva. Eur. J. Clin. Invest. 1972;2(6): 398-406.
Chicharro J.L., Lucia A., Perez M., Vaquero A.F., Urena R. Saliva composition and exercise. Sports Med. 1998;26(1):17-27.
Cadore E., Lhullier F., Brentano M., Silva E., Ambrosini M., Spinelli R. et al. Correlations between serum and salivary hormonal concentrations in response to resistance exercise. J. Sports Med. 2008;26(10):1067-72.
Sannikka E., Terho P., Suominen J., Santti R. Testosterone concentrations in human seminal plasma and saliva and its correlation with non-protein-bound and total testosterone levels in serum. Int. J. Androl. 1983;6(4):319-30.
Baum B.J. Neurotransmitter control of secretion. J. Dent. Res. 1987;66(1):628-32.
Bokemeyer C., Bondarenko I., Hartmann J.T., de Braud F., Schuch G., Zubel A. et al. Efficacy according to biomarker status of cetuximab plus FOLFOX-4 as first-line treatment for metastatic colorectal cancer: the OPUS study. Ann. Oncol. 2011;22(7):1535-46.
Ramos D., Martins E.G., Viana-Gomez D., Casimiro-Lopez G., Salerno V.P. Biomarkers of oxidative stress and tissue damage released by muscle and liver after a single bout of swimming exercise. Appl. Physiol. Nutr. Metab. 2013;38(5):507-11.
de Oliviera V.N., Bessa A., Lamounier R.P.M.S., de Santana M.G., de Mello M.T., Espindola F.S. Changes in the salivary biomarkers induced by an effort test. Int. J. Sports Med. 2010;31(6):377-81.
Turpeinen U., Hamalainen E. Determination of cortisol in serum, saliva and urine. Best Pract. Res. Clin. Endocrinol. Metab. 2013;27(6):795-801.
Heintze U., Birkhed D., Bjorn H. Secretion rate and buffer effect of resting and stimulated whole saliva as a function of age and sex. Swed. Dent. J. 1983;7(6):227-38.
Watanabe S., Dawes C. The effects of different foods and concentrations of citric acid on the flow rate of whole saliva in man. Arch. Oral. Biol. 1988;33(1):1-5.
Pilardeau P., Richalet J.P., Bouissou P., Garnier M., Vaysse J., Margo J.N. et al. Secretion salivaire et exercise physique. Med. Sport. 1992;66(3-4):111-4.
Bosch J.A., Ring C., de Geus E.J.C., Veerman E.C.I., Amerongen A.V.N. Stress and secretory immunity. Int. Rev. Neurobiol. 2002;52:213-53.
Bishop N.C., Walker G.J., Scanlon G.A., Richards S., Rogers E. Salivary IgA responses to prolonged intensive exercise following caffeine ingestion. Med. Sci. Sports Exerc. 2006;38(3):513-9.
Sari-Sarraf V., Reilly T., Doran D.A., Atkinson G. The effects of single and repeated bouts of soccer-specific exercise on salivary IgA. Arch. Oral. Biol. 2007;52(6):526-32.
Granger D.A., Weisz J.R., McCracken J.T., Kauneckis D., Ikeda S. Testosterone and conduct problems. J. Am. Child. Adolesc. Psychiatry. 1994;33(6):908.
Muramatsu Y., Takaesu Y. Oral health status related to subgingival bacterial flora and sex hormones in saliva during pregnancy. Bull. Tokyo Dent. Coll. 1994;35(3):139-51.
Bishop N.C., Gleeson M. Acute and chronic effects of exercise on markers of mucosal immunity. Front. Biosci. 2009;14(2):4444-56.
Guthrie R., Susi A. A simple phenylalanine method for detecting phenylketonuria in large populations of newborn infants. Pediatrics. 1963;32:338-43.
Pelton R. Bioactive paper provides a low-cost platform for diagnostics. Trends Analyt. Chem. 2009;28(8):925-42.
Clinical and laboratory standards institute. Blood collection on filter paper for newborn screening programs. Approved standard, 6th ed. NBS01-A6. Wayne, PA: Clinical and Laboratory Standards Institute; 2013.
Chen J., Hsieh Y. Stabilizing drug molecules in biological samples. Ther. Drug Monit. 2005;27(5):617-24.
Li W., Zhang J., Tse F.L.S. Strategies in quantitative LC-MS/MS analysis of unstable small molecules in biological matrices. Biomed. Chromatogr. 2011;25(1-2):258-77.
van Amsterdam P., Waldrop C. The application of dried blood spot sampling in global clinical trials. Bioanalysis. 2010;2(11):1783-6.
Fokkema M.R., Bakker A.J., de Boer F., Kooistra J., de Vries S., Wolthuis A. HbA1c measurements from dried blood spots: validation and patient satisfaction. Clin. Chem. Lab. Med. 2009;47(10):1259-64.
Leichtle A.B., Ceglarek U., Witzigmann H., Gabel G., Thiery J., Fiedler G.M. Potential of dried blood self-sampling for cyclosporine C2 monitoring in transplant outpatients. J. Transplant. 2010;6:1-6.
Alodaib A., Carpenter K., Wiley V., Sim K., Christodoulou J., Wilcken B. An improved ultra performance liquid chromatography-tandem mass spectrometry method for the determination of alloisoleucine and branched chain amino acids in dried blood samples. Ann. Clin. Biochem. 2011;48(5):468-70.
Merton G., Jones K., Lee M., Johnston A., Holt D.W. Accuracy of cyclosporin measurements made in capillary blood samples obtained by skin puncture. Ther. Drug Monit. 2000;22(5):594-8.
Woods K., Douketis J.D., Schnurr T., Kinnon K., Powers P., Crowther M.A. Patient preferences for capillary vs. venous INR determination in an anticoagulation clinic: a randomized controlled trial. Thromb. Res. 2004;114(3):161-5.
Mee J.M.L., Korth J., Halpern B. Rapid and quantitative blood analysis for free fatty acids by chemical ionization mass spectrometry. Anal. Lett. 1976;9(12):1075-83.
Chace D.H., Kalas T.A., Naylor E.W. Use of tandem mass spectrometry for multianalyte screening of dried blood specimens from newborns. Clin. Chem. 2003;49(11):1797-1817.
Thomas A., Geyer H., Guddat S., Schanzer W., Thevis M. Dried blood spots (DBS) for doping control analysis. Drug Test. Anal. 2011;3(11-12):806-13.
Tretzel L., Thomas A., Geyer H., Pop V., Schanzer W., Thevis M. Dried blood spots (DBS) in doping controls: a complementary matrix for improved in- and out-of-competition sports drug testing strategies. Anal. Methods. 2015;7(18):7596-7605.
Thevis M., Kuuranne T., Dib J., Thomas A., Geyer H. Do dried blood spots (DBS) have the potential to support result management processes in routine sports drug testing? Drug Test. Anal. 2020;12(6):704-10.
Lange T., Thomas A., Walpurgis K., Thevis M. Fully automated dried blood spot sample preparation enables the detection of lower molecular mass peptide and non-peptide doping agents by means of LC-HRMS. Anal. Bioanal. Chem. 2020;412(14):3765-77.
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