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DEVELOPMENT OF A PCR ASSAY FOR THE DETECTION OF HUMAN HERPES VIRUS TYPE 7


DOI: https://doi.org/10.51620/0869-2084-2022-67-11-658-662

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Abstract

A PCR assay has been developed to identify the DNA of the human herpes virus type 7. The search and selection of conserved regions was carried out by comparing the whole genome nucleotide sequences of HHV-7. A fragment duplicated in the HHV-7 genomes was chosen as a target for amplification. The performance of the assay was tested on a synthetic matrix and clinical samples. The developed assay has high sensitivity and specificity and showed good efficiency in detecting HHV-7 DNA in clinical samples.


Для цитирования: Марданлы С.С., Марданлы С.Г., Казаков А.А., Демкин В.В., Затевалов А.М., Миронов А.Ю. Разработка ПЦР-тест-системы для детекции вируса герпеса человека 7 типа. Клиническая лабораторная диагностика. 2022; 67(11): 658-662. https://doi.org/10.51620/0869-2084-2022-67-11-658-662

For citation: Mardanly S.S., Mardanly S.G., Kazakov A.A., Demkin V.V., Zatevalov A.M., Mironov A.Yu. Development of a PCR assay for the detection of human herpes virus type 7. Klinicheskaya Laboratornaya Diagnostika (Russian Clinical Laboratory Diagnostics). 2022; 67(11): 658-662 (in Russ.). https://doi.org/10.51620/0869-2084-2022-67-11-658-662

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