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DEVELOPMENT OF A REAGENT KIT FOR QUALITATIVE DETECTION OF SARS-COV-2 VIRUS RNA IN NASO AND OROPHARYNGEAL SMABS BY REAL-TIME RT-PCR


DOI: https://doi.org/10.51620/0869-2084-2022-67-11-663-667

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Abstract

The coronavirus infection continues to spread around the world. In this regard, the purpose of this work was: to develop a set of reagents for the qualitative detection of SARS-CoV-2 virus RNA. The set was developed by CJSC «Ecolab», 20 positive samples were used to develop the kit. The research method consisted of several stages: isolation of SARS-CoV-2 RNA, RNA reverse transcription reaction and PCR amplification of cDNA with simultaneous detection of the result in real time. The main characteristics of the kit: analytical sensitivity - 100%, specificity - 100%, accuracy - 100%. Thus, our method for diagnosing a new coronavirus infection based on real-time RT-PCR makes it possible to qualitatively and quickly detect betacoronavirus RNA in clinical material from patients and healthy individuals with suspected coronavirus infection and other symptoms of SARS.


Для цитирования: Безродный С.Л. Жигалева О.Н. Марданлы С.Г. Помазанов В.В. Гашенко Т.Ю. Разработка набора реагентов для качественного выявления РНК вируса SARS-CoV-2 в назо- и орофарингеальных мазках методом ОТ-ПЦР в режиме реального времени. Клиническая лабораторная диагностика. 2022; 67(11): 663-667. https://doi.org/10.51620/0869-2084-2022-67-11-663-667

For citation: Bezrodny S.L. Zhigaleva O.N. Mardanly S.G. Pomazanov V.V. Gashenko T.Yu. Development of a reagent kit for the qualitative detection of SARS-CoV-2 virus RNA in naso- and oropharyngeal swabs by real-time RT-PCR. Klinicheskaya Laboratornaya Diagnostika (Russian Clinical Laboratory Diagnostics). 2022; 67(11): 663-667 (in Russ.). https://doi.org/10.51620/0869-2084-2022-67-11-663-667

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