ASSOCIATIONS OF ALLELIC VARIANTS OF GH1 AND IGF2 GENES WITH DELAYED GROWTH AND MALNUTRITION IN NEWBORNS
The slow growth and fetal malnutrition are an important medical and social problem, but many mechanisms for the development of this pathology are unexplored. The aim of the study. To study the unfavorable allelic variants for the growth hormone (GH) and insulin-like growth factor-2 (IGF-2) genes in the intrauterine retardation growth and malnutrition in infants. Materials and Methods. 174 children were examined. Out of them, there were 46 cases underweighted for the gestational age (P05.0 for ICD-10) (1 group), and 83 patients were “small for gestational age “ (P05.1) (2nd group), 45 infants had no metabolic disorders (control). DNA was isolated from cord white blood cells. Single-nucleotide substitutions with the real-time detection of results were typed using the polymerase chain reaction. Results The deviation from the Hardy-Weinberg equilibrium was revealed only for the GH1: g.4995a> g in the group of healthy infants, mainly due to the difference in the observed and expected homozygosis from the minor allele. In 63% of the infants from the 1st group, the GH1-TA genotype g.6169t>a was detected against 44.4% in the control (p <0.05). Under the unfavorable allelic variants, GH1: g.4995a>g, in 52.1% of the newborns of the 1st group the heterozygous genotype was detected almost 2 times more often than in healthy infants (26.7%, p = 0.02). With the carriage of this genotype, the risk of prenatal weight deficit increased by 3 times (OR=3,0; 95% CI [1,25-7,22]). Under the unfavorable allelic variants GH1: g.4995a> g, in 47% of the 2 groups the heterozygous genotype of AG was detected, against 26.7% in the control (p<0.05). Conclusion An association of the heterozygous genotype GH1: g.4995a> g with “underweighted for the gestational age” has been identified.
About the authorsKocherova Victoria V.
Shcherbak V. A.
Slancheva B, Mumdzhiev H. Small for gestational age newborns-definition, etiology and neonatal treatment. Akush.Ginekol. (Sofia).2013; 52(2): 25-32.
Accrombessi M, Zeitlin J, Massougbodji A, Cot M, Briand V. What Do We Know about Risk Factors for Fetal Growth Restriction in Africa at the Time of Sustainable Development Goals? A Scoping Review. Paediatr Perinat Epidemiol. 2017; doi: 10.1111/ppe.12433.
Sania A, Smith E.R., Manji K., Duggan C., Masanja H., Kisenge R. et al. Neonatal and Infant Mortality Risk Associated with Preterm and Small for Gestational Age Births in Tanzania: Individual Level Pooled Analysis Using the Intergrowth Standard. J Pediatr. 2018; 192(1): 66-72.
Strizhakov A.N., Ignatko I.V., Timohina E.V, Belocerkovceva L.D. Fetal growth retardation syndrome: pathogenesis, diagnosis, treatment, obstetric tactics. [Sindrom zaderzhki rosta ploda: patogenez, diagnostika, lechenie, akusherskaja taktika]. Moscow; GEOTAR-Media», 2013. (In Russian)
Petrova IN, Kovalenko TV, Ermolaeva NN. Dynamics of the prevalence of intrauterine growth retardation in the Udmurt Republic. Trudy Izhevskoy gosudarstvennoy meditsinskoy akademii. Sbornik nauchnykh statey. Izhevsk, 2015; 86-7. (In Russian)
Sevruk O.V. Features of the neonatal period in full-term infants with intrauterine growth retardation. Vesti natsionalnoy akademii nauk Belarusi. 2012; 1: 14-9. (In Russian)
Ivanov DO, Kozlova LV, Derevcov VP. Neuropsychiatric development in children with intrauterine growth retardation in the first half of life. Pediatr. 2017; 8(1): 40-1. (In Russian)
Giabicani E, Pham A, Brioude F, Mitanchez D, Netchine I. Diagnosis and management of postnatal fetal growth restriction. Best Pract Res Clin Endocrinol Metab. 2018; 32(4): 523-34.
Phan Duy A. Intrauterine growth retardation and the developing brain. Arch. Pediatr. 2013; 20(9): 1034-8.
Kazantseva EV, Dogushkina NV. Modern aspects of pathogenesis, diagnosis and management tactics of pregnant women with fetal growth retardation syndrome. Zabaykal’skiy meditsinskiy vestnik. 2012; 2: 170-7. (In Russian)
Kocherova VV, Shcherbak VA. The somatotropic hormone and insulin-like factor II in newborns with intrauterine growth retardation and their mothers. Klinicheskaya laboratornaya diagnostika. 2017; 62(7). 422-5. (In Russian)
de Mascena Diniz Maia PF, da Silva TM, Andgelo HD, de Silva LW, Gondium Martins DB, de Mascena Diniz Maia M. et al. IGF2/ApaI polymorphism associated with birth weight in children of the region of Petrolina-PE. Brazil. J. Matern. Fetal. Neonatal. Med. 2013; 26(3): 316-7.
Adkins RM, Somes G, Morrison JC, Hill JB, Watson EM, Magann EF et al. J. Association of birth weight with polymorphisms in the IFG2, H19, and IGF2R genes. Pediatr Res. 2010; 68(5): 429-34.
Hăşmăşanu MG, Baizat M, Procopciuc LM, Blaga L, Văleanu MA, Drugan TC et al. Serum levels and ApaI polymorphism of insulin-like growth factor 2 on intrauterine growth restriction infants. Matern Fetal Neonatal Med. 2018; 31(11): 1470-6.
Calculator for calculating statistics in case-control studies [Electronic resource]. Mode: http: // gen-exp.ru.//calculator_or.php [Date 1 June, 2018] (In Russian)
Garford KL, Heinemann GK, Thompson SD, Buckberry S, Owens JA, Dekker GA. et al. SCOPE Consortium Circulation IGF 1 and IGF2 and SNP genotypes in men and pregnant and non-pregnant women. Endocr.connect. 2014; 3(3): 138-49.
Kolychev A.P. Insulin-like growth factor II (IGF-2). Among the regulatory peptides of insulin. Zhurnal evolyutsionnoy biokhimii i fiziologii. 2000; 36 (2): 69-82. (In Russian)
Osada H. Association between polymorphism in genes related to common adult diseases and fetal growth. Clinical Medicine: Pediatrics. 2009; 3; 11-8.
Kirilenko M.Yu. Study of growth factor gene polymorphisms with the development of primary open-angle glaucoma. Meditsinskie nauki, Fundamental’nye issledovaniya. 2013; 12: 222-25. (In Russian)
Potulova SV, Glotov OS, Baranov VS. Analysis of polymorphisms of IGF-1 and PGC-1 genes involved in energy metabolism in two age groups of St. Petersburg population. Ekologicheskaya genetika cheloveka. 2009; 7(1): 12-8. (In Russian)
Silvano L, Miras M, Perez A, Picotto G, Diaz de Barboza G, Munoz L. et al. Comparative analysis of clinical, biochemical and genetic aspects associated with bone mineral density in small for gestational age children. J Pediatr Endocrinol Metab. 2011; 24(7-8): 511-7.
Burris H H, Byan HM, Braun JM, Teller Rojo Solis MM. Association between birth and DNA methylation of IGF2, glucocorticoid receptor and repetitive elements LINE-1 and Alu. Epigenomics. 2013; 5(3): 271-81.
Kocherova V.V, Shcherbak V.A, Strambovskaya N.N, Dutova A.A. Polymorphism of genes regulating growth (GH1:119 T>G (rs 2005172), H1:T>A (rs2665802), GH1:-68A>G (rs1805086) and IGF2:13790 C>G (rs3213221)) mothers give birth to underweight children. Zabaykal’skiy meditsinskiy vestnik. 2017; 1: 44-8. (In Russian)
- Refbacks are not listed
Контент доступен под лицензией Creative Commons Attribution 3.0 License.