THE CYTOGENETIC AND PHYTOTOXIC ASSESSMENT OF THE ANTI-ICING MATERIAL ON ONION ASSAY (ALLIUM CEPA)

Allium cepa assay is one of the relevant methods for the cytogenetic analysis. Today modern anti-icing reagents seem to be unstudied in the field of cytogenetics studies. The aim of the experiment is to study the phytotoxic and cytogenetic activity of multi-component anti-icing material using an onion Allium cepa. We researched anti-icing concentrations in the range of from 1 to 100 g/l. The test object was onion «Stuttgarter risen» variety couched in anti-icing solutions during the 72 hours. According to the assessment of phytotoxic effects, there was estimated the number of roots, the length of the longest root and the length of all roots for the every onion plant. The parallel experiment included the phytotoxic research of the same anti-icing using wheat seeds according to method MR 2.1.7.2297-07. Every onion roots were cut away, fixed, painted and pressured for microscopic analysis for the purposes of the cytogenetic research. About 2000 cells were analyzed for each onion. We estimated the number of mitosis per 1000 cells (mitotic index, ‰) and the proportion of cells in each phase of mitosis by the total number of dividing cells (%). For the analysis of cytogenetic damage using micronucleus test that provided the estimation of micronucleus, protrusions, and binuclear cells. An ana-telophase analysis included the estimation of fragments bridges and lagging chromosomes. As a result of analysis of the roots growth, we founded acting concentrations (2; 4; 10 and 100 g/l) and in-acting ones (1.3 and 1). The experiment showed the similar susceptibility of the onion and wheat root response. The mitosis analysis showed that concentrations from 1 to 1.3 g/l increased a mitosis activity and concentration of 10 g/l provided the decline of mitosis. There were no cytogenetic defects in all studied concentrations.

Grant W.F. Chromosome aberration assays in Allium. A report of U.S. environmental protection agency gene-tox program. Mutat. Res. 1982: 99(3): 273-91.

WHO monographs on selected medicinal plants. World Health Organization: Geneva; 1999.

Islamutdinova A.A., Shayakhmetov A.I., Gaydukova I.V. Allium-test and the mathematical model in the assessment of cyclic ammonium compounds toxicity. Aktual’nye problemy gumanitarnykh i estestvennykh nauk. 2012; (3): 51-7. (in Russian)

Tkachuk N.V., Yanchenko V.A., Demchenko A.M. Antibacterial and phytotoxic activity of derivatives of antipyrine with imidazoazepine fragment. Vestnik BGU. Seriya 2: Khimiya. Biologiya. Geografiya. 2015; (1): 38-43. (in Russian)

Ul’yanenko L.N., Synzynys B.I., Amosova N.V., Netsvet N.V. Detoxification of aluminum ions of iron and other interactions between the metal ions in plant. In: Chemistry, Biology and Biotechnologies: challenges and prospects for development. Collected materials of the international scientific e-symposium, Moscow, Russia, on 26-28 June 2014 [Khimiya, biologiya i biotekhnologii: problemy i perspektivy razvitiya. Sbornik materialov mezhdunarodnogo nauchnogo e-simpoziuma, Rossiya, g. Moskva, 26-28 June 2014]. Kirov: Mezhdunarodnyy tsentr nauchno-issledovatel’skikh proektov; 2014: 46-61. (in Russian)

Emirova D.E. Screening of phytotoxic effect of pesticide bi-58 with use of allium cepa l. and zea mays l Magazine. Chelovek-priroda-obshchestvo: teoriya i praktika bezopasnosti zhiznedeyatel’nosti, ekologii i valeologii. 2010; (3): 60-3. (in Russian)

Zotov A.V., Kudryavtseva E.A., Kavelenova L.M. By assessing the allelopathic and genotoxic activity of leaf litter of some exotic species of wood. Vestnik molodykh uchenykh i spetsialistov Samarskogo gosudarstvennogo universiteta. 2013; (2): 17-23. (in Russian)

Pesnya D.S., Serov D.A., Vakorin S.A., Prokhorova I.M. Research of the toxic, mitosis modifying and mutagen effect of Heracleum Sosnowskyi. Yaroslavskiy pedagogicheskiy vestnik. 2011; 3(4): 93-8. (in Russian)

Pesnya D.S., Romanovskiy A.V., Prokhorova I.M. Investigation of toxic, mitotoxic and mutagenic effects of synthesized food dyes by the Allium test. Yaroslavskiy pedagogicheskiy vestnik. 2012; 3(3): 86-93. (in Russian)

Kudryavtsev A.A., Mikhaylova L.V., Rybina G.E., Gordeeva F.V., Tsulaiya A.L., Znamenshchikov A.N. The study of migration and degradation of oil in peat soil of upland bogs in KHMAO under control of biotesting. Sibirskiy ekologicheskiy zhurnal. 2012; (6): 761-8. (in Russian)

Ibragimova E.E. Mitotical activity of cells of root meristems of allium cepa l. At the united action of pesticides and heavy metals. Uchenye zapiski Tavricheskogo natsional’nogo universiteta imeni V.I. Vernadskogo. Seriya: Biologiya. Khimiya. 2014: 27(66): 56-63. (in Russian)

Pyatkova S.V., Geras’kin S.A., Vasil’eva A.N., Koz’min G.V., Lyannoy N.N. Features of using an allium-test to evaluate the toxicity of water and soil samples with radioactively contaminated areas. Izvestiya vysshikh uchebnykh zavedeniy. Yadernaya energetika. 2009; (3): 50-7. (in Russian)

Bagdasaryan A.S. Efficiency test systems for evaluating the toxicity of natural media. Ekologiya i promyshlennost’ Rossii. 2007; (1): 44-8. (in Russian)

Kontsevaya I.I., Tolkacheva T.A. Improving bioassay technique based Allium test. Vestnik Vitebskogo gosudarstvennogo universiteta. 2012; (6): 57-65. (in Russian)

Kryatov I.A., Tonkopiy N.I., Vodyanova M.A., Rusakov N.V., Doner’yan L.G., Evseeva I.S., Ushakov D.I., Matveeva I.S., Vorob’eva O.V., Tsapkova N.N. Methodological approaches to the substantiation of hygienic requirements to application of deicing materials. Gigiena i sanitariya. 2014; 93(6): 52-4. (in Russian)

Chudakova S.B. Toxicological and hygienic evaluation of the degree of danger anti-icing reagents [Toksikologo-gigienicheskaya otsenka stepeni opasnosti antigololednykh reagentov]. Diss. Moscow; 2006. (in Russian)

Sbitnev A.V., Vodyanova M.A., Kryatov I.A., Doner’yan L.G., Evseeva I.S., Ushakova O.V., Ushakov D.I., Matveeva I.S., Rodionova O.M. Methodological aspects of the assessment of phytotoxicic properties of ice-melter reagents. Gigiena i sanitariya. 2016; 95(8): 52-4. (in Russian)

Pukhal’skiy V.A., Solov’ev A.A., Badaeva E.D., Yurtsev V.N. Workshop on Cytology and cytogenetics of plants [Praktikum po tsitologii i tsitogenetiki rasteniy]. Moscow: KolosS; 2007. (in Russian)

Sycheva L.P. The biological significance, and the criteria for determining the full range of variation chapels karyological indicators when assessing the human cytogenetic status. Meditsinskaya genetika. 2007; 6(11): 3-11. (in Russian)