CONTROL OF THE POLLUTION OF ATMOSPHERIC AIR UNDER THE USE OF PESTICIDES ON THE BASIS OF TRIAZOLINTION

Introduction. The analytical decision on control of active ingredient of pesticides of a chemical class of a triazolintione - the prothioconazole considered as the system fungicide of the new generation possessing protective, eradicating and medical action in atmospheric air is presented in the article. Material and Methods. The method is based on HPLC with the UV-detector (wavelength of detecting of 213 nm), includes a sampling of the air environment on paper filters “blue film” with a speed of aspiration of 5 l/min. The extraction of a prothioconazole from filters is carried out an acetonitrile. For the concentrating of the extract from filters, there was used the reception of SPE with the application of cartridges on the basis of C18. It is noted that concentrating cannot be executed directly without preliminary dilution of an aliquot of the extract by water in a volume ratio of 1:9. Because of features of this active ingredient, his tendency to degradation for stabilization of the received water solutions amino acid cysteine is used. Results. The linearity of the calibration characteristic is confirmed in the range of concentration of 0.05 - 0.5 µ/ml (correlation coefficient more than 0.999). The lower limit of quantitation of a prothioconazole in the air environment amounts to 0.0025 mg/m3 under the aspiration of 80 L of air that is 8 times lower than the established border level of a prothioconazole in atmospheric air (0.02 mg/m3). The total error of the measurement does not exceed 16%. The developed method was applied for the determination of prothioconazole’ exposure levels in natural conditions in samples of atmospheric air within a sanitary gap taken of processing of spraying of field cultures, preliminary processing of seeds of grain, soy, corn, and potatoes, aircraft processing of field cultures (sunflower).

Pesticide Chemistry. Crop Protection, Public Health, Environmental Safety. Ed. by Ohkawa H., Miyagawa H., Lee P.W. Verlag: WILEY-VCH; 2007: 497.

The Pesticide Manual. 17th Edition, Turner J.A., еd. Alton: BCPS; 2015.

Haidukowski M., Perrone G., Visconti A., et al. Effect of prothioconazole-based fungicides on Fusarium head blight, grain yield and deoxynivalenol accumulation in wheat under field conditions. Phytopathologia Mediterranea. 2012; 51 (1): 236-46. Available at: http://www.fupress.net/index.php/pm/article/view/9401

Paul P.A., Lipps P.E., Hershman D.E., et al. Efficacy of triazole-based fungicides for Fusarium head blight and deoxynivalenol control in wheat: a multivariate meta-analysis. Phytopathology. 2008; 98: 999-1011. Available at: https://www.ncbi.nlm.nih.gov/pubmed/18943738

Treikale О., Afanasieva I., Pugacheva E. Protection of winter wheat against head blight using a new fungicide Prozaro. Plant protection and quarantine. 2011; 6: 49-50 (in Russian).

Baybakova Ye. V., Nefedieva Ye. E., Khokhlova T.V. The effect of prothioconazole on wheat physiological properties. Subtropical and ornamental gardening. 2017; 61: 138-41 (in Russian).

Evaluation of the new active prothioconazole in the product redigo fungicidal seed treatment. Australian Pesticides and Veterinary Medicines Authority. Australia: Canberra; 2007. Available at: https://apvma.gov.au/sites/default/files/publication/13941-prs-prothioconazole.pdf

Parker J.E., Warrilow A.G.S., Cools H.J., Martel C.M., Nes W.D., Fraaije B.A., et al. Mechanism of Binding of Prothioconazole to Mycosphaerella graminicola CYP51 Differs from That of Other Azole Antifungals. Appl Environ Microbiol. 2011; 77(4): 1460-5.

Beloshapkina O.O., Akimov T.A. Complex evaluation of fungicide treatments of winter wheat seeds in field and in vitro trials. Theoretical and applied problems of agro-industrial complex. 2016; 1 (26): 58-64 (in Russian)

Komkov N.D. New fungicide “Bayer Cropscience” - the key to get good harvest. Plant protection and quarantine. 2010; 5: 36-7 (in Russian).

Shcherbakov P.A. Lamador: excellent start and successful finish of an agricultural season. Plant protection and quarantine. 2010; 3: 76-7 (in Russian)

The reference book of the pesticides and agrochemicals allowed for use in the territory of the Russian Federation, Moscow: LLC Publishing House Agrorus, 2017. Year-book. Release 21 (in Russian).

Haas M., Justus K. Metabolism of Prothioconazole (JAU6476) in animals and plants. Pflanzenschutz-Nachr. Bayer - English edition. 2004; 57(2): 207-24. Available at: http://www.cnshb.ru/jour/j_as.asp?id=18231

Baybakova E.V., Nefedyeva E.E., Belopukhov of S.L. Issledovaniye of influence of modern protravitel on viability and growth of sprouts of grain crops. News of higher education institutions. Applied chemistry and biotechnology. 2016; 6 (3): 57-64 (in Russian).

Parker J.E.,Warrilow A.G., Cools H.J., Fraaije B.A., Lucas J.A., Rigdova K.,et al. Prothioconazole and Prothioconazole-Desthio Activities against Candida albicans Sterol 14-α-Demethylase. Appl Environ Microbiol. 2013 Mar; 79(5): 1639-45. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591943

Beyer M., M.B. Klix and J.A. Verreet. Quantifying the effects of previous crop, tillage, cultivar and triazole fungicides on the deoxynivalenol content of wheat grain - a review. Journal of Plant Diseases and Protection. 2006; 113: 241-6. Available at: http://wiki.pestinfo.org/wiki/Journal_of_Plant_Diseases_and_Protection_(2006)_113,_241-246

Muravyeva S. I., Bukovsky M. I., Prokhorov E. K. et al. Guidelines of the control of hazardous substances in workplace air: Ref. ed. M.: Chemistry; 1991 (in Russian).

Hellpointner E., Borchers H. Behaviour of Prothioconazole (JAU 6476) in the environment. Pflanzenschutz-Nachr.Bayer - English edition. 2004; 57 (2): 163-80.

Font G., Manes J., Molto J.C., Pico Y. Solid-phase extraction in multi-residue pesticide analysis of water. J. Chromatograph. Anal. 1993; 642: 135-61. Available at: http://arch.neicon.ru/xmlui/handle/123456789/1693630?show=full

Sychev K.S., Davankov V.A. Materials and methods of sample preparation in chromatography: solid phase concentration and adsorption purification. Sorption and chromatographic processes. 2004; 4 (1): 5-28 (in Russian)

Wu J., Tragas C., Lord H., Pawliszyn J. Analysis of polar pesticides in water and wine samples by automated intube solid-phase microextraction coupled with high-performance liquid chromatography-mass spectrometry. J. Chromatograph. Anal. 2002. 976; 1-2: 357-67. Available at: https://www.sciencedirect.com/science/article/pii/S0021967302010725

S.A. Barker. Matrix solid-phase dispersion. J. Chromat. A. 2000; 885: 115-27.

Deineka V.I., Deineka L.A., Sidorov A.N. et al. The evaluation of the properties of the solid-phase extraction cartridge sorbents: the role of the «gallery» pores. Sorption and chromatographic processes. 2016; 16 (5): 624-30 (in Russian).

Van Seeventer P.B., Weenen H., Winkel C., Kerler J. Stability of thiols in an aqueous process flavoring. 2001; 49: 4292-5. Available at: http://www.biomedsearch.com/nih/Stability-thiols-in-aqueous-process/11559126.html

Wang C.I.A., Harvey P.J., Lewis R.J. Stabilization of the cysteine-rich conotoxin mria by using a 1,2,3-triazole as a disulfide bond mimetic. Angewandte chemie - international edition. 2015; 54 (4): 1361-4.

Weerawatanakorn M., Wu J.-Ch., Pan M.-Hs, Ho Ch.-T. Reactivity and stability of selected flavor compounds. J. Food and Drug Anal. 2015; 23 (2): 176-90. Available at: https://www.sciencedirect.com/science/article/pii/S1021949815000277

Charles-Bernard M., Roberts D.D., Kraehenbuehl K. Interactions between volatile and nonvolatile coffee components. 2. Mechanistic study focused on volatile thiols. J Agric Food Chem. 2005; 53: 4426-33. Available at: https://www.scopus.com/record/display.uri?eid=2-s2.0-20744454899&origin=inward&txGid=99b59e2e41903ccf7cf67c3e05faadc4

Cortez R., Luna-Vital D.A., Margulis D., Gonzalez de Mejia E. Natural Pigments: Stabilization Methods of Anthocyanins for Food Applications. Comprehensive reviews in Food Science and Food Safety. 2017; 16 (1): 180-98. Available at: http://onlinelibrary.wiley.com/doi/10.1111/1541-4337.12244/full

Sandmann A., Kompch A., Mackert V., Liebscher Ch. L., Winterer M. Interaction of l-Cysteine with ZnO: Structure, Surface Chemistry, and Optical Properties. Langmuir. 2015; 31 (21): 5701-11. Available at: https://pubs.acs.org/doi/abs/10.1021/la504968m

Zheng J., Yang T., Zhou J., Xu M., Zhang X., Rao Zh. Elimination of a Free Cysteine by Creation of a Disulfide Bond Increases the Activity and Stability of Candida boidinii Formate Dehydrogenase. Appl. Environ. Microbiol. 2017; 83 (2). Available at: http://aem.asm.org/content/83/2/e02624-16.full.