EXPERIMENTAL STUDY OF THE IMPACT OF LOW FLUORINE CONCENTRATIONS ON THE TISSUE LEVEL OF HSP FAMILY PROTEINS
Introduction. Fluoride in high concentrations has a toxic effect not only on bone tissue but also on the heart, liver, kidneys, and brain. In the implementation of the response to toxic doses of fluorine the proteins of the HSP family are involved regulating intracellular and tissue homeostasis under various stress effects. The toxic effect of high fluorine concentrations the mechanisms of which are disclosed in fluorosis can be realized and at a level significantly lower than a toxic one. In the literature, there is little data on the peculiarities of the effects of low fluorine concentrations at the tissue and cellular levels. The aim of the study. To investigate the impact of low fluorine concentrations on the tissue level of HSP family proteins in the brain and liver of laboratory animals. Material and methods. The experiments were carried out on 60 white male rats of the same age weighing 200-250 g. The rats were divided into 2 groups: the control and the group of the animals exposed to sodium fluoride (NaF) within 6 weeks (at a concentration of 10 mg/l corresponding to the daily fluorine dose of 1.2 mg/kg per body weight). We determined the level of inducible HSP72 and HSP32 (heme-oxygenase-1) referred to proteins of HSP family (Heat shock proteins), the activity of free radical processes and antioxidant enzymes (superoxide dismutase (SOD) and catalase) in the brain and liver tissues. Results. The important role of stress-inducible HSP72 protein in protecting the brain from the damage caused by the prolonged exposure to low fluorine concentrations was shown. In the liver, a protective role against fluoride exposure is played by the protein HSP32 with antioxidant properties. At the tissue level, the prolongation of the terms of the development of chronic fluoride intoxication with low fluorine concentrations was revealed. In the liver appeared to be the highly sensitive organ to the fluorine accumulation, the significant lesion was detected.
About the authorsZhukova Anna G.
Agalakova N.I., Gusev G.P. Effect of inorganic fluoride on living organisms of different phylogenetic level. Zhurnal evolyutsionnoy biokhimii i fiziologii. 2011; 47 (5): 337-47. (in Russian)
Cicek E., Aydin G., Akdogan M., Okutan H. Effects of chronic ingestion of sodium fluoride on myocardium in a second generation of rats. Hum. Exp. Toxicol. 2005; 24 (2): 79-87.
Chouhan S., Lomash V., Flora S.J.S. Fluoride-induced changes in haem biosynthesis pathway, neurological variables and tissue histopathology of rats. J. Appl. Toxicol. 2010; 30 (1): 63-73.
Barbier O., Arreola-Mendoza L., Del Razo L.M. Molecular mechanisms of fluoride toxicity. Chem. Biol. Interact. 2010; 188 (2): 319-33. Doi: 10.1016/j.cbi.2010.07.011
Shalina T.I., Vasilyeva L.S. General problems of toxic effect of fluorine. Sibirskiy meditsinskiy zhurnal. 2009; 88 (5): 5-9. (in Russian)
Lee M., Arikawa K., Nagahama F. Micromolar levels of sodium fluoride promote osteoblast differentiation through Runx2 signaling. Biol. Trace Elem. Res. 2017. DOI 10.1007/s12011-017-0930-5
Zhou Z., Wang H., Zheng B., Han Zh., Chen Ya., Ma Yan. A rat experimental study of the relationship between fluoride exposure and sensitive biomarkers. Biol. Trace Elem. Res. 2017; 180 (1): 100-9.
Panneerselvam L., Raghundth A., Perumal E. Differential expression of myocardial heat shock proteins in rats acutely exposed to fluoride. Cell Stress & Chaperones. 2017; 22 (5): 743-50.
Rosly O.F., Gurvich V.B., Plotko E.G., Kuzmin S.V., Fedoruk A.A., Roslaya N.A. et al. Emerging issues concerning hygiene in the Russian aluminum industry. Meditsina truda i promyshlennaya ekologiya. 2012; (11): 8-12. (in Russian)
Roslaya N.A., Likhachyova E.I., Oransky I.E., Odinokaya V.A., Plotko E.G., Zhovtyak E.P. et al. Clinical and pathogenetic aspects of the chronic occupational intoxication with fluorine compounds in modern reality. Meditsina truda i promyshlennaya ekologiya. 2012; (11): 17-22. (in Russian)
Fridovich I. Superoxide radical and superoxide dismutases. Accounts Chem. Res. 1972; 5: 321-6. DOI: 10.1021/ar50058a001
Luck H. Catalase. In: Bergmeyer H.U., ed. Methods of enzymatic analysis. New York: Verlag-Chemie Academic Press; 1963: 885-88.
Archipenko Yu.V., Didenko V.V., Sazontova T.G., Meerson F.Z. Comparative evaluation of the effect of immobilization stress on the dynamics of resistance to induction of lipid peroxidation of internal organs and brain. Doklady AN SSSR. 1989; 304 (6): 1500-503. (in Russian)
Lankin V.Z., Tikhaze A.K., Konovalova G.G. et al. Concentration inversion of antioxidant and prooxidant action of β-carotene in tissues in vivo. Byulleten’ eksperimental’noy biologii i meditsiny. 1999; 128 (9): 314-8. (in Russian)
Ohkawa H., Ohishi N., Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1979; 95 (2): 351-8.
Kikugava K., Kojima T., Yamaki S. et al. Interpretation of the thiobarbituric acid reactivity of rat liver and brain homogenates in the presence of ferric ion and ethylenediaminetetraacetic acid. Anal. Biochem. 1992; 202: 249-55.
Reddy K.P., Sailaja G., Krishnaiah C. Protective effects of selenium on fluoride induced alterations in certain enzymes in brain of mice. J Environ Biol. 2009; 30 (5 Suppl.): 859-64.
Chattopadhyay A., Podder S., Agarwal S. Fluoride-induced histopathology and synthesis of stress protein in liver and kidney of mice. Arch Toxicol. 2010; 85 (4): 327-35.
Chen Q., Wang Z., Xiong Y., Xue W., Kao X., Gao Y. et al. Selenium increases expression of HSP70 and antioxidant enzymes to lesser oxidative damage in Fincoal-type fluorosis. J. Toxicol. Sci. 2009; 34 (4): 399-405.
Basha M.P., Sujitha N.S. Chronic fluoride toxicity and myocardial damage: antioxidant offered protection in second generation rats. Toxicol. Int. 2011; 18 (2): 99-104.
Zhukova A.G., Alekhina D.A., Sazontova T.G. et al. Mechanisms of intracellular defense and activity of free radical oxidation in rat myocardium in the dynamics of chronic fluorine intoxication. Byulleten’ eksperimental’noy biologii i meditsiny. 2013; 156 (2): 224-7. (in Russian)
Andreeva L.I., Boykova A.A., Margulis B.A. Peculiarities of intracellular content and functional role of heat shock proteins 70 kDa under stress and adaptation. Tekhnologii zhivykh sistem. 2009; 6 (3): 11-7. (in Russian)
Tarakanov I.A., Tikhomirova L.N., Zhukova A.G., Safonov V.A. The resistance of low brainstem tissue to free radical oxidation in rats during periodic breathing following hydroxybutyrate treatment. Patol Fiziol Eksp Ter. 2013; (4): 21-5. (in Russian)
Reddy P.H., Reddy T.P., Manczak M. et al. Dynamin-Related Protein 1 and Mitochondrial Fragmentation in Neurodegenerative Diseases. Brain Res. Rev. 2011; 67(1-2): 103-18. Doi: 10.1016/j.brainresrev.2010.11.004.
Tarakanov I.A., Tikhomirova L.N., Zhukova A.G., Safina N.F. Pro- and Antioxidant Systems in the Lower Portion of Rat Brainstem during Hydroxybutyrate-Induced Pathological Periodic Breathing. Byulleten’ eksperimental’noy biologii i meditsiny. 2016; 162 (1):14-7.
Zhukova A.G., Sazontova T.G. Hemeoxygenase: function, regulation, biological role. Hypoxia Medical Journal. 2004; 12 (3): 30-43.
Evdonin A.L., Medvedeva N.D. The extracellular heat shock protein 70 and its functions. Cytology. 2009; 51 (2): 130-7. (in Russian)
Hong J-M., Lee S-M. Heme oxygenase-1 protects liver against ischemia/reperfusion injury via phosphoglycerate mutase family member 5-mediated mitochondrial quality control. Life Sciences. 2018; (200): 94-104. Doi.org/10.1016/j.lfs.2018.03.017.
Zhukova A.G., Mikhailova N.N., Yadykina T.K., Alekhina D.A., Gorokhova L.G., Romanenko D.V. et al. Experimental studies of intracellular liver protective mechanisms in development of chronic fluorine intoxication. Meditsina truda i promyshlennaya ekologiya. 2016; (5): 21-4. (in Russian)
Alekhina DA, Zhukova AG, Sazontova TG. Low dose of fluoride influences to free radical oxidation and intracellular protective systems in heart, lung and liver. Technologies of living systems. 2016; 13 (6): 49-56. (In Russian)
- Refbacks are not listed
Контент доступен под лицензией Creative Commons Attribution 3.0 License.